Liver Histological Response of Female Broodstock Catfish to Dietary Soybean Meal Supplementation
DOI:
https://doi.org/10.31938/jsn.v16i2.970Keywords:
catfish, soybean meal, liver histology, vacuolization, hepatocytesAbstract
Feed is one of the key factors in fish culture, including the use of plant-based ingredients such as soybean meal as an alternative protein source that may affect the physiological condition of organs, particularly the liver. This study aimed to analyse the effect of soybean meal supplementation in feed on the liver histological response of catfish (Clarias sp.). Liver histological characteristics were evaluated in three treatment groups: a control group (without soybean meal supplementation), 5% soybean meal-supplemented diet, and 10% soybean meal-supplemented diet. The results showed that the control group exhibited a relatively normal liver histological structure, characterised by an orderly arrangement of hepatocytes, centrally located nuclei, and clearly visible sinusoids. In the 5% soybean meal treatment, more pronounced histological alterations were observed, particularly cytoplasmic vacuolization of hepatocytes, indicating moderate to severe steatosis. Meanwhile, hepatocyte vacuolization was also observed in the 10% soybean meal treatment, but with a severity ranging from mild to moderate compared with the 5% treatment. These findings indicate that dietary soybean meal supplementation affects liver histology in catfish through a non-linear response pattern. The 5% dose caused greater tissue alterations than the 10% dose, which may be associated with the adaptive response of hepatocytes to feed exposure. These findings indicate that the use of soybean meal in fish feed should consider an appropriate dosage to maintain liver health.
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osomatic index (GSI), hepatosomatic index (HSI), and spawning success of female broodstock catfish. Nekton, 5(2), 177–185. https://doi.org/10.47767/nekton.v5i2.1066
Nuzaiba, P. M., Varghese, T., Gupta, S., Sahu, N. P., & Srivastava, P. P. (2022). Estrogenic and vitellogenic responses in genistein fed adult male Cyprinus carpio. Aquaculture, 548, 1-11. https://doi.org/10.1016/j.aquaculture.2021.737559
Pang, A., Wang, T., Xie, R., Wang, Z., Xin, Y., Tan, B., & Zhang, W. (2024). Effects of soybean meal on immunity and transcriptomics of liver in pearl gentian grouper (Epinephelus fuscoguttatus ? × Epinephelus lanceolatus ?). Aquaculture Reports, 35. https://doi.org/10.1016/j.aqrep.2024.101969
Pousis, C., Rodríguez, C., De Ruvo, P., De Virgilio, C., Pérez, J. A., Mylonas, C. C., Zupa, R., Passantino, L., Santamaria, N., Valentini, L., & Corriero, A. (2019). Vitellogenin receptor and fatty acid profiles of individual lipid classes of oocytes from wild and captive-reared greater amberjack (Seriola dumerili) during the reproductive cycle. Theriogenology, 140, 73–83. https://doi.org/10.1016/j.theriogenology.2019.08.014
Qin, P., Wang, T., & Luo, Y. (2022). A review on plant-based proteins from soybean: Health benefits and soy product development. Journal of Agriculture and Food Research, 7. https://doi.org/10.1016/j.jafr.2021.100265
Sun, S. X., Liu, Y. C., Limbu, S. M., Li, D. L., Chen, L. Q., Zhang, M. L., Yin, Z., & Du, Z. Y. (2023). Vitellogenin 1 is essential for fish reproduction by transporting DHA-containing phosphatidylcholine from liver to ovary. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1868(4). https://doi.org/10.1016/j.bbalip.2023.159289
Tan, S. T., Tan, S. S., & Tan, C. X. (2023). Soy protein, bioactive peptides, and isoflavones: A review of their safety and health benefits. In PharmaNutrition (Vol. 25). Elsevier B.V. https://doi.org/10.1016/j.phanu.2023.100352
Tramunt, B., Montagner, A., Tan, N. S., Gourdy, P., Rémignon, H., & Wahli, W. (2021). Roles of estrogens in the healthy and diseased oviparous vertebrate liver. In Metabolites (Vol. 11, Number 502). MDPI AG. https://doi.org/10.3390/metabo11080502
Wolf, J. C., & Wheeler, J. R. (2018). A critical review of histopathological findings associated with endocrine and non-endocrine hepatic toxicity in fish models. In Aquatic Toxicology (Vol. 197, pp. 60–78). Elsevier B.V. https://doi.org/10.1016/j.aquatox.2018.01.013
Ye, Z., Zhao, T., Wei, Q., Lin, H., Zhang, Y., & Li, S. (2022). Distinct roles of estrogen receptors in the regulation of vitellogenin expression in orange-spotted grouper (Epinephelus coioides). International Journal of Molecular Sciences, 23(8362). https://doi.org/10.3390/ijms23158632
Zhai, H. F., Tian, Y. N., Sheng, Y. X., Pu, Y. J., Gao, Y. R., Chen, J. Y., Liu, J. Di, Ma, J., Xu, H. M., Yang, P. Bin, & Li, H. M. (2025). Study on hepatotoxicity of benzophenone-3 at environmental concentration in postpartum mice. Toxics, 13(1014). https://doi.org/10.3390/toxics13121014
Zhao, Z., Peng, L., Zhao, Q., & Wang, Z. (2024). Genome-wide identification, expression and response to estrogen of vitellogenin gene family in sichuan bream (Sinibrama taeniatus). International Journal of Molecular Sciences, 25(6379). https://doi.org/10.3390/ijms25126739
Zhao, Z., Zhao, Q., Wang, H., Wei, L., Wang, S., Li, S., Yuan, D., & Wang, Z. (2023). Integrated transcriptomic and metabolomic analyses identify key factors in the vitellogenesis of juvenile sichuan bream (Sinibrama taeniatus). Frontiers in Marine Science, 10, 1–6. https://doi.org/10.3389/fmars.2023.124376
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Copyright (c) 2026 VIKA MAULIDIYAH, Rizky Kusma Pratiwi, Muhamad Dwi Cahya, Zakiyyah Nur Inayah, Vina Nur Nadiro, Anissa Aprilia Nurkhasanah, Maura Yasmin Dhia, Fajar Maulana Akmal Firdaus
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